Phytochemical Screening and Determination of Total Flavonoid Content of Matoa (Pometia pinnata) Leaf Extract Using UV-Vis Spectrophotometry Method

Fitriani Damayanti

Fitriani Damayanti Universitas Muslim Indonesia

Keywords: Matoa (Pometia pinnata) Leaf; Extraction; Phytochemical Screening; Flavonoid Content; UV-Vis Spectrophotometry

Abstract

ABSTRACT

Matoa (Pometia pinnata) is a plant belonging to the Sapindaceae family that spreads in the tropical country, including Indonesia. Almost all parts of the plant are utilized by the community including leaves, stem bark, fruit bark and roots which have potential as medicinal plants. Empirically, matoa leaves (Pometia pinnata) can be used as a medicine for fever, skin pain and swollen sprains. Matoa (Pometia pinnata) leaf cooking water can relieve hypertension. Matoa (Pometia pinnata) plants are known to contain flavonoid compounds. Where flavonoid compounds are reported to have antioxidant, antibacterial, antiallergic, antiviral, and anticancer activities. This study aims to determine the phytochemical compound group and determine the flavonoid content of matoa (Pometia pinnata) leaves from the Gowa Regency, South Sulawesi Province. The extract was obtained through maceration method with 96% ethanol solvent. After the thick extract was obtained, phytochemical screening test and determination of flavonoid content by UV-Vis spectrophotometry with quercetin as the standard. Based on the results of this study it can be concluded that matoa leaf extract (Pometia pinnata) contains a class of flavonoids, tanins, saponins, and triterpenoid compounds. Meanwhile, the results of the study showed that the total flavonoid content of matoa leaf extract (Pometia pinnata) at a wavelength of 430 nm was 15,884 mg QE/g extract or 1.588,4%.

Keywords : Matoa (Pometia pinnata) Leaf; Extraction; Phytochemical Screening; Flavonoid Content; UV-Vis Spectrophotometry

References

[1] Zaki PH, Gandaseca S, Rashidi NM, Ismail MH. Traditional Usage Of Medicinal Plants By Temiar Tribes In The State Of Kelantan, Peninsular Malaysia. Journal Forest And Society. 2019;3(November):227–234.
[2] Ziraluo YPB. Tanaman Obat Keluarga Dalam Perspektif Masyarakat Transisi (Studi Etnografis Pada Masyarakat Desa Bawodobara). Jurnal Inovasi Penelitian. 2020;1(3):1–4.
[3] Rahmawati, Tahir M, Wulandari AH. Kandungan Senyawa Kimia Dan Aktivitas Farmakologi Tanaman Matoa (Pometia Pinnata J.R. Forster & J.G. Forster). Jurnal Farmasi. 2021;13(2):108–115.
[4] Maryam F, Taebe B, Toding DP. Pengukuran Parameter Spesifik Dan Non Spesifik Ekstrak Etanol Daun. Jurnal Mandala Pharmacon Indonesia. 2020;6(1):1–12.
[5] Sidoretno WM, Fauzana A. Aktivitas Antioksidan Daun Matoa (Pometia Pinnata) Dengan Variasi Suhu Pengeringan. Indonesia Natural Research Pharmaceutical Journal. 2018;3(1):2502–8421.
[6] Darwis. Formulasi dan Uji Aktivitas Antioksidan Ekstrak Daun Matoa (Pometia pinnata) dengan Metode DPPH. Jurnal Ilmiah Fitomedika Indonesiai. 2022;1(1):19-25.
[7] Wahyulianingsih, Handayani S, Malik A. Penetapan Kadar Flavonoid Total Ekstrak Daun Cengkeh. Jurnal Fitofarmaka Indonesia. 2016;3(2).
[8] Ikalinus R, Widyastuti SK, Setiasih NL. Skrining Fitokimia Ekstrak Etanol Kulit Batang Kelor (Moringa Oleifera). Indonesia Medicus Veterinus. 2015;4(1):77.
[9] Rossalinda, Wijayanti F, Iskandar D. Effectiveness Of Matoa Leaf (Pometia Pinnata) Extract As An Antibacterial Staphylococcus Epidermidis. Stannum : Jurnal Sains Dan Terapan Kimia. 2021;3(1):1–8.
[10] Syamsul ES, Hakim YY, Nushasnawati H. Penetapan Kadar Flavonoid Ekstrak Daun Kelakai (Stenochlaena palustris (Burm. F.) Bedd.) Dengan Metode Spektrofotometri Uv-Vis. Jurnal Riset Kefarmasian Indonesia. 2019;1(1):11–20.

[11] Yulianti R, Dahlia A, Ahmad AR. Penetapan Kadar Flavonoid Total Dari Ekstrak Etanolik Daun Benalu Mangga. Jurnal Fitofarmaka Indonesia. 2014;1(1):14–17.
[12] Wendersteyt NV, Wewengkang DS, Abdullah SS. Uji Aktivitas Antimikroba dari Ekstrak dan Fraksi Ascidian Herdmania momus dari Perairan Pulau Bangka Likupang Terhadap Pertumbuhan Candida albicans. Pharmacon. 2021;10(1):706.
[13] Ahmad AR, Juwita, Ratulangi SAD, Malik A. Penetapan Kadar Fenolik dan Flavonoid Total Ekstrak Metanol Buah dan Daun Patikala (Etlingera elatior (Jack) R.M.SM). Journal Pharmaceutical Sciences and Research. 2015;2(1):1-10.
[14] Malik A, Edward F, Waris R. Skrining Fitokimia dan Penetapan Kandungan Flavonoid Total Ekstrak Metanolik Herba Boroco (Celosia argentea L.). Jurnal Fitofarmaka Indonesia. 2014;1(1):1-5.
[15] Martiningsih NW, Widana GAB, Kristiyanti PLP, Bandyopadhyay S, Mukerji J, Yenerel NM, Dinc UA, Gorgun E, Radical F, Activity S, Alsophila OF, Sm J, Zuhra CF, Tarigan JB, Sihotang H. Skrining Fitokimia Dan Uji Aktivitas Antioksidan Ekstrak Etanol Daun Matoa (Pometia Pinnata) Dengan Metode Dpph. Journal Of Ocular Pharmacology And Therapeutics. 2016;3(3):332–338.
[16] Arsul MI, Tahar N, Rauf A. Analisis Kualitatif dan Kuantitatif Antioksidan Parang Romang. Jurnal Sains Dan Kesehatan. 2022;4(4):379–385.
[17] Zirconia A, Kurniasih N, Amalia V. Identifikasi Senyawa Flavonoid Dari Daun Kembang Bulan (Tithonia diversifolia) Dengan Metode Pereaksi Geser. Al-Kimiya. 2015;2(1):9–17.
[18] Forestryana D, Arnida. Skrining Fitokimia Dan Analisis Kromatografi Lapis Tipis Ekstrak Etanol Daun Jeruju (Hydrolea Spinosa L.). Jurnal Ilmiah Farmako Bahari. 2020;11(2):113.
[19] Aminah, Tomayahu N, Abidin Z. Penetapan Kadar Flavonoid Total Ekstrak Etanol Kulit Buah Alpukat (Persea americana Mill.) dengan Metode Spektrofotometri UV-Vis. Jurnal Fitofarmaka Indonesia. 2017;4(2):226–230.
[20] Mustika I, Indrawati A, Warsyidah AA. Uji Efektifitas Biji Kelor (Moringa Oleifera) Terhadap Penurunan Kadar Besi (Fe) Air Sumur Gali Di Desa Buhung Bundang Kecamatan Bontotiro Kabupaten Bulukumba. Jurnal Media Laboran. 2018;8(1):9–14.
[21] Purnamasari A, Zelviani S, Sahara S, Fuadi N. Analisis Nilai Absorbansi Kadar Flavonoid Tanaman Herbal Menggunakan Spektrofotometer Uv-Vis. Teknosains: Media Informasi Sains Dan Teknologi. 2022;16(1):57–64.
[22] Fadillah A, Rahmadani A, Rijai L. Analysis of total flavonoid and antioxidant activity of Passion leaves (Passiflora foetida L.). Proceeding of the 5th Mulawarman Pharmaceuticals Conference. 2017:23–24.
[23] Lindawati NY, Ma’ruf SH. Penetapan Kadar Total Flavonoid Ekstrak Etanol Kacang Merah (Phaseolus Vulgaris L.) Secara Spektrofotometri Visibel'. Jurnal Ilmiah Manuntung. 2020;6(1):83.
[24] Nuryadin Y, Naid T, Dahlia AA, Dali S. Kadar Flavonoid Total Ekstrak Etanol Daun Serai Dapur dan Daun Alang-Alang Menggunakan Spektrofotometri UV-Vis. Jurnal Kesehatan. 2018;1(4):337-345.