The DETERMINATION OF TANNIN CONTENT OF AVOCADO (Persea americana Mill). SEED ETHANOL EXTRACT USING THE UV-VIS SPECTROPHOTOMETRY METHOD

Rahmatullah Basri

Rahmatullah Basri Universitas Muslim Indonesia

Keywords: Avocado Seeds (Persea americana Mill.); ethanol; Tannins; UV-Vis Spectrophotometry.

Abstract

Avocado (Persea americana Mill.) is a plant that grows in most tropical and sub-tropical countries, one of which is in Indonesia. Avocado seeds (Persea americana Mill.) contain phytochemical compounds, one of which is tannin which has anti-inlammatory properties. This study aims to determine the levels of tannins in ethanol extract of avocado (Persea americana Mill.) seeds. The tannins in avocado seeds were extracted using the maceration method with 96% ethanol solvent. In the qualitative test and quantitative test, in the qualitative test using FeCl₃reagent and in the quantitative test the determination of tannin content in the ethanol extract of avocado seeds was measured using UV-Vis spectrophotometry at a wavelength of 770 nm with tannic acid as a comparison. The results showed that the average tannin content of the ethanol extract of avocado seeds at a concentration of 500 ppm was 69,237 mgTAE/g extract.

References

[1] Tinesya D, Andhita N, Vidmar R. Ekspolarasi Potensi Ekstrak Biji Alpukat (Persea americana) Sebagai Agen Antiinflamasi. J Pendidik IPA. 2019;9(2):52–6.
[2] Fatonah R, Mulyaningsih S, Ardiana C. Penentuan Kadar Total Tanin Dari Ekstrak Daun Binahong (Anredera cordifolia). J Life Sci. 2021;3(2):53–65.
[3] Mustarichie R, Runadi D. Isolation and identification of flavonoids from avocado leaves (Persea americana Mill). Asian J Pharm Dev. 2021;9(6):34–40.
[4] Hidayah N. Pemanfaatan Senyawa Metabolit Sekunder Tanaman (Tanin dan Saponin) dalam Mengurangi Emisi Metan Ternak Ruminansia. J Sain Peternak Indones. 2016;11(2):89–98.
[5] Desinta T. Penentuan Jenis Tanin Secara Kualitatif dan Penetapan Kadar Tanin dari Kulit Buah Rambutan (Nephelium lappaceum L.) Secara Permanganometri. J Ilm Mhs Univ Surabaya. 2015;4(1):1–10.
[6] Ojha S, Raj A, Roy A, Roy S. Extraction of total phenolics, Flavonoids and Tannins from Paederia foetida L. Leaves and their Relation with Antioxidant Activity. Pharmacogn J. 2018;10(3):541–7.
[7] Nonci FY. Penetapan Kadar Tanin Total Ekstrak Biji Jintan Hitam ( Nigella sativa ) Secara Spektrofotometri UV-ViS. JF FIK UINAM. 2014;2(4):154–8.
[8] Pratama M, Razak R, Rosalina VS. Analisis Kadar Tanin Total Ekstrak Etanol Bunga Cengkeh (Syzygium aromaticum L.) Menggunakan Metode Spektrofotometri UV-ViS. J Fitofarmaka Indones. 2019;6(2):368–73.
[9] Susanty, Bachmid F. Perbandingan Metode Ekstraksi Maserasi Dan Refluks Terhadap Kadar Fenolik Dari Ekstrak Tongkol Jagung (Zea mays L.). J Konversi. 2016;5(2):87–93.
[10] Nurhasnawati H, Sukarmi, Handayani F. Perbandingan Metode Ekstraksi Maserasi Dan Sokletasi Terhadap Aktivitas Antioksidan Ekstrak Etanol Daun Jambu Bol (Syzygium malaccense L.). J Ilm Manuntung. 2017;3(1):91.
[11] Sarlina S, Razak AR, Tandah MR. Uji Aktivitas Antibakteri Sediaan Gel Ekstrak Daun Sereh (Cymbopogon nardus L. Rendle) terhadap Bakteri Staphylococcus aureus Penyebab Jerawat. J Farm Galen (Galenika J Pharmacy). 2017;3(2):143–9.
[12] Amelia FR. Penentuan Jenis Tanin Dan Penetapan Kadar Tanin Dari Buah Bungur Muda (Lagerstroemia speciosa Pers.) Secara Spektrofotometri Dan Permanganometri. J Ilm Mhs Univ Suabaya. 2015;4(2):1–20.
[13] Ergina, Nuryanti S, Pursitasari ID. Uji Kualitatif Senyawa Metabolit Sekunder Pada Daun Palado ( Agave angustifolia ) Yang Diekstraksi dengan Pelarut Air dan Etanol. J Akad Kim. 2014;3(3):165–72.